Diabetes increases inflammation and lung injury associated with protective ventilation strategy in mice

作者：Xiong Xiang qing; Wang Wan tie; Wang Liang rong; Jin Li da; Lin Li na^*

来源：International Immunopharmacology, 2012, 13(3): 280-283.

DOI：10.1016/j.intimp.2012.04.020

• Mechanical ventilation
• Low tidal volume
• Inflammation
• Diabetes
• Lung injury

Background: Mechanical ventilation may paradoxically cause lung injury. Protective mechanical ventilation strategy utilizing low tidal volume and high frequency has been shown to attenuate inflammation and reduce mortality in non-diabetic patients. The purpose of this present study was to observe the effects of diabetes on inflammation and lung injury in mice with protective ventilation strategy.
Methods: Forty mice were included in our study. The mice in Group Dia-MV and Con-MV were subjected to 4 hour-ventilation. And the mice in Group Dia-SB and Con-SB were exposed to room air breathing spontaneously for 4 h. Tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), interleukin-10 (IL-10), superoxide dismutase (SOD) and malondialdehyde (MDA) levels in serum were detected and the expression of inflammatory cytokine mRNA was also determined in lung tissue. Lung damage was assessed using a modified lung injury score.
Results: The serum levels of TNF-alpha, IL-6, and IL-10 in Group Dia-MV were significantly higher than those in Group Dia-SB or Group Con-MV or Group Con-SB (P<0.05). Quantitative RT-PCR analysis of pro-inflammatory cytokines in lung homogenates presented similar results. The mice in Group Dia-MV suffered obvious lung histological changes, whose lung injury scores were significantly higher in Group Dia-SB as compared to Group Con-SB, Group Con-MV or Group Dia-SB (P<0.05).
Conclusions: Diabetes increased the inflammation reaction and associated lung injury in mice in spite of the protective mechanical ventilation strategy based on low tidal volumes and high frequency.

• 出版日期2012-7
• 单位温州医科大学