Migration-inducing gene-7 independently predicts poor prognosis of human osteosarcoma and is associated with vasculogenic mimicry

作者：Ren, Ke; Zhang, Jian; Gu, Xiaojie; Wu, Sujia; Shi, Xin; Ni, Yicheng; Chen, Yong; Lu, Jun; Gao, Zengxin; Wang, Chen^*; Yao, Nan^*

来源：Experimental Cell Research, 2018, 369(1): 80-89.

DOI：10.1016/j.yexcr.2018.05.008

• Osteosarcoma
• Migration-inducing gene-7
• Vasculogenic mimicry
• Prognosis
• Metastasis

Vasculogenic mimicry (VM) is a special type of vascular channel formed by tumor cells without endothelial cell participation. Migration-inducing gene 7 (MIG-7) plays an important role in regulating VM. In this study, immunohistochemical staining was used to detect MIG-7 in tissue specimens from 141 primary osteosarcoma patients, and the relationship between MIG-7 and VM was examined. Survival analysis were performed to evaluate the prognoses. MIG-7 knockdown osteosarcoma cells were used for cell proliferation, apoptosis, migration, invasiveness and VM formation assays. A spontaneously metastasizing cell line-derived orthotopic xenograft mouse model was established to evaluate the effect of MIG-7 knockdown on tumorigenesis, VM formation and lung metastasis. MIG-7 expression was associated with VM formation. There were significant differences in overall and metastasis-free survival between the MIG-7-positive and MIG-7-negative groups. The MIG-7 expression was shown to be an independent indicator of both overall and metastasis-free survival. In vitro knockdown of MIG-7 dramatically reduced migration, invasion and VM formation in osteosarcoma cells without any significant effect on cell proliferation and apoptosis. MIG-7 knockdown also exhibited potent antitumor, antimetastasis and anti-VM effects in the orthotopic mouse model of 143B osteosarcoma. Therefore, MIG-7 serves as an independent unfavorable prognostic indicator in osteosarcoma patients and MIG-7 is an important mediator of osteosarcoma VM formation.

• 出版日期2018-8-1
• 单位大连交通大学; 南京大学; 东南大学; 南京中医药大学; 江苏省中医药研究院