MicroRNA-520a-3p suppresses non-small-cell lung carcinoma by inhibition of High Mobility Group on 1 (HMGB1)

作者：Lv, X.; Ya, L.; Nie, Y. -Q.; Xu, X. -Y.^*

来源：European Review for Medical and Pharmacological Sciences, 2018, 22(6): 1700-1708.

• Non-small-cell lung carcinoma
• microRNA-520a-3p
• HMGB1
• Tumorigenesis

OBJECTIVE: Currently, pathogenesis of non-small cell lung carcinoma (NSCLC) is still unknown and the treatment is far from ideal. Therefore, we investigated the effect of inhibiting microRNA-520a-3p in NSCLC cells.
MATERIALS AND METHODS: NCI-H157 cells were treated with microRNA-520a-3p analogs for 48 h, or microRNA-520a-3p analogs and its inhibitor, for a total of 48 h. Many tests were performed, including MTT, flow cytometry, wound healing assay and transwell assay. The tumor model was established, and HMGB1 mRNA was detected by RT-PCR. Protein levels of HMGB1, MMP-2, MMP-9, BcI-2, Bax, and Caspase-3 were assessed by Western Blot.
RESULTS: microRNA-520a-3p could significantly inhibit the proliferation, migration and invasion of NCI-H157 cells, inducing their apoptosis. microRNA-520a-3p inhibited tumor growth and decreased the mRNA and protein levels of HMGB1. Additionally, it decreased the Bol-2/Bax ratio, MMP-2 and MMP-9 expression, and increased caspase-3 expression.
CONCLUSIONS: microRNA-520a-3p exhibited an effective inhibition on NCI-H157 tumor growth likely by inhibiting HMGB1 expression.

• 出版日期2018-3
• 单位临沂市人民医院