Development of a blocking ELISA using a recombinant glycoprotein for the detection of antibodies to vesicular stomatitis New Jersey virus

作者：Heo Eun Jeong; Lee Hyang Sim; Jeoung Hye Young; Ko Hyo Rim; Kweon Chang Hee; Ko Young Joon^*

来源：Journal of Virological Methods, 2010, 164(1-2): 96-100.

DOI：10.1016/j.jviromet.2009.12.005

• Vesicular stomatitis New Jersey virus
• Recombinant glycoprotein
• Blocking ELISA

A recombinant glycoprotein (R-GP) of vesicular stomatitis New Jersey virus (VSV-NJ) was expressed in insect cells by a baculovirus system. Its utility as a diagnostic antigen in a blocking ELISA was investigated as an alternative to the current native GP extracted from VSV-NJ. With the cut-off value of 73% inhibition, the R-GP ELISA exhibited 99.1% specificity for naive sera from cattle and horses. It did not cross-react with VSV-Indiana (VSV-IN) positive sera and differentiated from foot-and-mouth disease and swine vesicular disease. Taken together, this is the first report that the R-GP has a potential to be used as a diagnostic antigen in place of the native GP for the detection of antibodies to VSV-NJ in cattle and horses. 2009 Published by Elsevier B.V.

• 出版日期2010-3