Direct electrochemistry of hemoglobin on graphene/Fe3O4 nanocomposite-modified glass carbon electrode and its sensitive detection for hydrogen peroxide

作者：Wang Yuqiao^*; Zhang Huijun; Yao Dan; Pu Junjie; Zhang Yuan; Gao Xiaorui; Sun Yueming

来源：Journal of Solid State Electrochemistry, 2013, 17(3): 881-887.

DOI：10.1007/s10008-012-1939-5

• Direct electrochemistry
• Nanocomposite
• Sensitive detection
• Hydrogen peroxide

Graphene/Fe3O4 nanocomposite was prepared for the immobilization of hemoglobin (Hb) to improve the electron transfer between Hb and glass carbon electrode (GCE). The characterization of nanocomposites was described by transmission electron microscopy, Fourier transform infrared, Raman spectroscopy, and X-ray photoelectron spectroscopy, respectively. The electrochemistry of Hb on the graphene/Fe3O4-based GCE was investigated by cyclic voltammetry and amperometric measurement. The modified electrode showed a wide linear range from 0.25 mu mol/L to 1.7 mmol/L with a correlation coefficient of 0.9967. The detection limit of the H2O2 biosensor was estimated at 6.0 x 10(-6) mol/L at a signal-to-noise ratio of 3.

• 出版日期2013-3
• 单位河北工程大学; 东南大学; 扬州大学