Apoptotic markers in protozoan parasites

作者：Jimenez Ruiz Antonio^*; Fernando Alzate Juan; MacLeod Ewan Thomas; Lueder Carsten Guenter Kurt; Fasel Nicolas; Hurd Hilary

来源：Parasites & Vectors, 2010, 3(1): 104.

DOI：10.1186/1756-3305-3-104

The execution of the apoptotic death program in metazoans is characterized by a sequence of morphological and biochemical changes that include cell shrinkage, presentation of phosphatidylserine at the cell surface, mitochondrial alterations, chromatin condensation, nuclear fragmentation, membrane blebbing and the formation of apoptotic bodies. Methodologies for measuring apoptosis are based on these markers. Except for membrane blebbing and formation of apoptotic bodies, all other events have been observed in most protozoan parasites undergoing cell death. However, while techniques exist to detect these markers, they are often optimised for metazoan cells and therefore may not pick up subtle differences between the events occurring in unicellular organisms and multicellular organisms.
In this review we discuss the markers most frequently used to analyze cell death in protozoan parasites, paying special attention to changes in cell morphology, mitochondrial activity, chromatin structure and plasma membrane structure/permeability. Regarding classical regulators/executors of apoptosis, we have reviewed the present knowledge of caspase-like and nuclease activities.

• 出版日期2010-11-9
• 单位河北医科大学