Approach toward an efficient inoculum preparation stage for suspension BHK-21 cell culture

作者：Fernandez Nunez Eutimio Gustavo; Leme Jaci; Parizotto Leticia de Almeida; de Rezende Alexandre Goncalves; Vale da Costa Bruno Labate; Lopes Boldorini Vera Lucia; Calil Jorge Soraia Attie; Astray Renato Mancini; Pereira Carlos Augusto; Caricati Celso Pereira; Tonso Aldo

来源：Cytotechnology, 2016, 68(1): 95-104.

DOI：10.1007/s10616-014-9756-6

• Inoculum preparation
• Mammalian cells
• BHK-21
• Static cell culture
• Suspension cell culture
• Spinner flask
• Bioreactor

Mammalian cells are the most frequently used hosts for biopharmaceutical proteins manufacturing. Inoculum quality is a key element for establishing an efficient bioconversion process. The main objective in inoculation expansion process is to generate large volume of viable cells in the shortest time. The aim of this paper was to optimize the inoculum preparation stage of baby hamster kidney (BHK)-21 cells for suspension cultures in benchtop bioreactors, by means of a combination of static and agitated culture systems. Critical parameters for static (liquid column height: 5, 10, 15 mm) and agitated (working volume: 35, 50, 65 mL, inoculum volume percentage: 10, 30 % and agitation speed: 25, 60 rpm) cultures were study in T-flask and spinner flask, respectively. The optimal liquid column height was 5 mm for static culture. The maximum viable cell concentration in spinner flask cultures was reached with 50 mL working volume and the inoculum volume percentage was not significant in the range under study (10-30 %) at 25 rpm agitation. Agitation speed at 60 rpm did not change the main kinetic parameters with respect to those observed for 25 rpm. These results allowed for a schedule to produce more than 4 x 10(9) BHK-21 cells from 4 x 10(6) cells in 13 day with 1,051 mL culture medium.

• 出版日期2016-1