Antioxidant and Antiproliferation Effects of Extractable and Nonextractable Polyphenols Isolated from Apple Waste Using Different Extraction Methods

作者：Tow Wei Wei; Premier Robert; Jing Hao; Ajlouni Said^*

来源：Journal of Food Science, 2011, 76(7): T163-T172.

DOI：10.1111/j.1750-3841.2011.02314.x

• ABTS
• antiproliferation
• DPPH
• extractable polyphenols
• nonextractable polyphenols

Fruits and vegetables waste byproducts could be utilized as a good source of cheap antioxidants for improving human health and reducing the risks of some chronic diseases. Results from examining industrial apple waste revealed that the total polyphenolic content in nonextractable polyphenols (NEPPs) reached 539.84 +/- 8.90 mg as gallic acid equivalents/1 g of dry wt. in comparison to 77.26 +/- 11.53 mg dry wt. of extractable polyphenols (EPPs). The antioxidant activities of NEPPs reported as percentage reduction in 2,2-Diphenyl-1-picrylhydrazyl and 2'.2'-azinobis(3-ethylbenzothiazline-6-sulfonic acid) diammonium salt were 89.76 +/- 0.93% and 99.78 +/- 0.38%, respectively. In addition, the antiproliferation study on human HeLa, HepG2, and HT-29 cancer cells showed that NEPPs at the concentration of 1 mg/mL had significant inhibitory effects against all tested cancer cells (46.2% to 95%), where EPP showed lower effect (3.9% to 22.2%). These results clearly indicated that NEPPs from industrial apple waste could be a good source of natural antioxidants with significant antiproliferation efficacy against human cancer cells.

• 出版日期2011-9
• 单位中国农业大学