Identification and Characterization of Carbon-Centered Free Radicals Formed from Lipoxygenase-Catalyzed Lipid Peroxidation of Dihomo-gamma-linolenic Acid

作者：Yu Qing Feng; Xiao Ying; Ni Kun Yi; Qian Steven Y^*

来源：Chinese Journal of Analytical Chemistry, 2009, 37(12): 1815-1819.

• Dihomo-gamma-linolenic acic
• lipid peroxidation
• spins trapping
• liquid chromatography/electron spin resonance
• liquid chromatography/mass spectrometry

A combination technique of LC/ESR, LC/MS and spin trapping was used to identify and characterize the carbon-centered free radicals formed from lipoxygenase-catalyzed peroxidation of dihomo-gamma-linolenic acid (DGLA). The spin trap, alpha-[4-pyridyl-1-oxide]-N-tert-butyl nitrone (POBN), Could react with short-lived carbon-centered radicals to form relatively stable adducts. Based oil the same retention time of POBN radical. adduct in LC/UV/ESR and LC/MS, molecular weight of adduct could be confirmed and the structure of adduct could be confirmed by their LC/MS(2) fragment pattern. The results showed that free radicals formed in lipoxygenase-catalyzed peroxidation of DGLA. Including (center dot)C(10)H(13)O(2) (center dot) C(10)H(17)O(2) and (center dot) C(5)H(11) all stemmed from beta-scission of DGLA alkoxyl radicals (8-, 11-, 15-LO(center dot)). The results were helpful for further study 01' biological activities of these radicals in vivo.

• 出版日期2009-12
• 单位中国药科大学