Diet intervention reduces uptake of alpha(v)beta(3) integrin-targeted PET tracer F-18-galacto-RGD in mouse atherosclerotic plaques

作者：Saraste Antti; Laitinen Iina; Weidl Eliane; Wildgruber Moritz; Weber Axel W; Nekolla Stephan G; Hoelzlwimmer Gabriele; Esposito Irene; Walch Axel; Leppanen Pia; Lisinen Irina; Luppa Peter B; Yla Herttuala Seppo; Wester Hans Juergen; Knuuti Juhani; Schwaiger Markus^*

来源：Journal of Nuclear Cardiology, 2012, 19(4): 775-784.

DOI：10.1007/s12350-012-9554-5

• Atherosclerosis
• PET
• inflammation
• molecular imaging
• vulnerable plaque

Expression of alpha(v)beta(3) integrin has been proposed as a marker for atherosclerotic lesion inflammation. We studied whether diet intervention reduces uptake of alpha(v)beta(3) integrin-targeted positron emission tomography tracer F-18-galacto-RGD in mouse atherosclerotic plaques. %26lt;br%26gt;Hypercholesterolemic LDLR-/- ApoB(100/100) mice on high-fat diet for 4 months were randomized to further 3 months on high-fat diet (high-fat group, n = 8) or regular mouse chow (intervention group, n = 7). Intima-media ratio describing plaque burden was comparable between intervention and high-fat groups (2.0 +/- A 0.5 vs 2.3 +/- A 0.8, P = .5). Uptake of F-18-galacto-RGD in the aorta was lower in the intervention than high-fat group (%ID/g 0.16 vs 0.23, P %26lt; .01). Autoradiography showed 35% lower uptake of F-18-galacto-RGD in the atherosclerotic plaques in the intervention than high-fat group (P = .007). Uptake of F-18-galacto-RGD in plaques correlated with uptake of H-3-deoxyglucose and nuclear density, which was lower in the intervention than high-fat group (P = .01). Flow cytometry demonstrated macrophages expressing alpha(v) and beta(3) integrins in the aorta. %26lt;br%26gt;Uptake of F-18-galacto-RGD in mouse atherosclerotic lesions was reduced by lipid-lowering diet intervention. Expression of alpha(v)beta(3) integrin is a potential target for evaluation of therapy response in atherosclerosis.

• 出版日期2012-8