Anticancer Activity of Pterostilbene in Human Ovarian Cancer Cell Lines

作者：Pei, Hui-lin; Mu, Dan-mei; Zhang, Bin^*

来源：MEDICAL SCIENCE MONITOR, 2017, 23: 3192-3199.

DOI：10.12659/MSM.901833

• Cytokines
• Ovarian Neoplasms
• Tumor Necrosis Factor-alpha

Background: Epithelial ovarian cancer is a major cause of mortality in women and one of the most common gynecologic disorders. Pterostilbene (PTS), a trans-3,5-dimethoxy-4'-hydroxystilbene, was chosen for this work due to its reported effectiveness as a chemotherapeutic agent in cancer studies. In this work, we studied underlying molecular mechanisms of PTS treatment in various ovarian cancer cell lines such as OVCAR8, OV1063, IGROV-1, and SKOV3.
Material/Methods: We used the cytometric bead array (CBA) method and real-time PCR analysis to analyze the secretion level of tumor necrosis factor alpha (TNF-alpha) and to measure the TNF-alpha mRNA expression. NF-kappa B (NF-kappa B) promoter analysis, Western blot analysis, electrophoresis mobility shift assay (EMSA), and immunostaining analyses were performed to measure the NF-kB activity and other relative proteins levels.
Results: The PTS treatment decreased the release of TNF-alpha in IGROV-1 ovarian cancer cells. It also showed significant inhibitory effect on nuclear NF-kappa B p50, and NF-kappa B p65 protein levels.
Conclusions: From the results obtained, we suggest that PTS has the potential to treat ovarian cancer by reducing the level of TNF-a cytokine and to have a limited effect on NF-kappa B, AKT, and ERK signaling pathways.

• 出版日期2017-6-30
• 单位大庆油田总医院