Novosphingobium aquaticum sp nov., isolated from lake water in Suwon, Republic of Korea

作者：Singh Hina; Du Juan; Yang Jung Eun; Yin ChangShik; Kook MooChang; Yi Tae Hoo

来源：Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, 2015, 108(4): 851-858.

DOI：10.1007/s10482-015-0539-7

• Novosphingobium aquaticum
• Ubiquinone Q-10
• Gram-stain negative
• 16S rRNA

A novel Gram-stain negative, yellow coloured, strictly aerobic, rod-shaped, non-motile bacterium designated as THW-SA1(T), was isolated from lake water near Samsung apartment, Suwon, Republic of Korea. The phylogenetic analysis based on 16S rRNA gene sequences showed that strain THW-SA1(T) belongs to the genus Novosphingobium and is closely related to Novosphingobium taihuense (97.8 %) and Novosphingobium subterraneum (97.1 %). The DNA-DNA relatedness values between strain THW-SA1(T) and the most closely related type strains were found to be less than 30.0 %. The DNA G+C content was determined to be 67.5 mol%. The strain grows optimally at 25-28 A degrees C, at pH 7.0, and in the presence of 0.5 % NaCl. The predominant isoprenoid quinone was identified as ubiquinone Q-10. The polar lipid profile comprises diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipid, phosphatidylcholine, some unidentified phospholipids and some unidentified polar lipids. Fatty acids characteristic for this genus, such as C-16:1, C-14:0 2-OH, C-16:1 omega 6c and/or C-16:1 omega 7c (summed feature 3) and C-18:1 omega 6c and/or C-18:1 omega 7c (summed feature 8) were also detected. On the basis of the phenotypic and genotypic analysis, the strain THW-SA1(T) is considered to represent a novel species of the genus Novosphingobium, for which the name Novosphingobium aquaticum sp. nov. is proposed. The type strain is THW-SA1(T) (=KCTC 42608(T)=CCTCC AB 2015114(T)).

• 出版日期2015-10