A Combined Assay of hTERT and E6 Oncoprotein to Identify Virus-Infected Keratinocytes With Higher Telomerase Activity in Human Papillomaviruses 16 and 18-Related Bowenoid Papulosis

作者：Li, Dongsheng; Dong, Bilin; Hu, Zhimin; Chen, Liuqing; Zeng, Xianyu; Chen, Jinbo; Duan, Yiqun^*

来源：American Journal of Dermatopathology, 2012, 34(8): 813-817.

DOI：10.1097/DAD.0b013e318252f956

• bowenoid papulosis
• human papillomavirus
• human telomerase reverse transcriptase
• E6 oncoprotein
• screening

In the present study, we aim to evaluate the application potential of a combined assay of human telomerase reverse transcriptase (hTERT) and E6 oncoprotein in screening the virus-infected keratinocytes with higher telomerase activity in human papillomaviruses (HPV) 16- and 18-related bowenoid papulosis (BP). HPV16/18 DNA in BP (n = 123) was identified by in situ hybridization, the expression of hTERT and E6 in HPV16/18-related BP (n = 68) was determined by immunohistochemistry. We demonstrated that the expression of hTERT correlated well with that of E6 oncoprotein in HPV16/18-related BP lesions (Spearman rho = 0.868, P < 0.01). Furthermore, the majority of keratinocytes with positive nuclear staining for hTERT or E6 in the consecutive sections of each HPV16/18-related BP lesion showed nuclear paleomorphism or nuclear mitosis. In conclusion, we suggested that a combined assay of hTERT and E6 oncoprotein can be used to screen the HPV-infected keratinocytes with higher telomerase activity in HPV16-related and HPV18-related BP lesions.

• 出版日期2012-12
• 单位武汉市中西医结合医院