A selective novel non-enzyme glucose amperometric biosensor based on lectin-sugar binding on thionine modified electrode

作者：Li, Feng; Feng, Yan; Yang, Limin; Li, Liang; Tang, Chenfei; Tang, Bo^*

来源：Biosensors and Bioelectronics, 2011, 26(5): 2489-2494.

DOI：10.1016/j.bios.2010.10.040

• Thionine
• Concanavalin A
• Polydopamine
• D-Glucose
• Amperometric biosensor

A novel non-enzyme glucose amperometric biosensor was fabricated based on biospecific binding affinity of concanavalin A (Con A) for D-glucose on thionine (TH) modified electrode. TH can be covalently immobilized on potentiostatically activated glassy carbon electrode through Schiff-base reaction. Subsequently, the surface-adherent polydopamine film formed by self-polymerization of dopamine attached to TH and afforded binding sites for the subsequent immobilization of Con A molecules via Michael addition and/or Schiff-base reaction with high stability. Thus, a sensing platform for specific detection towards D-glucose was established. The binding of Con A towards D-glucose can be monitored through the decrease of the electrode response of the TH moiety. Due to the high affinity of Con A for 0-glucose and high stability of the resulting sensing platform, the fabricated biosensor exhibited high selectivity, good sensitivity, and wide linear range from 1.0 x 10(-6) to 1.0 x 10(-4) M with a low detection limit of 7.5 x 10(-7) M towards D-glucose.

• 出版日期2011-1-15
• 单位山东师范大学; 青岛科技大学