A Novel and Effective Strategy for the Isolation of Adipose-Derived Stem Cells: Minimally Manipulated Adipose-Derived Stem Cells for More Rapid and Safe Stem Cell Therapy

作者:Raposio Edoardo*; Caruana Giorgia; Bonomini Sabrina; Libondi Guido
来源:Plastic and Reconstructive Surgery, 2014, 133(6): 1406-1409.
DOI:10.1097/PRS.0000000000000170

摘要

Adipose-derived stem cells are an ideal mesenchymal stem cell population for regenerative medical application. The isolation procedure is performed by mechanical isolation under a laminar air flow bench without using serum or animal-derived reagents; cells were characterized by flow cytometric analysis. Cell availability is improved compared with enzymatic digestion procedures. The adipose-derived stem cell mechanical isolating procedure presented here is easier, safer, cheaper, and faster than traditional currently performed enzymatic procedures.

  • 出版日期2014-6