A pseudogene long-noncoding-RNA network regulates PTEN transcription and translation in human cells

作者：Johnsson, Per; Ackley, Amanda; Vidarsdottir, Linda; Lui, Weng-Onn; Corcoran, Martin; Grander, Dan; Morris, Kevin V.^*

来源：Nature Structural & Molecular Biology, 2013, 20(4): 440-+.

DOI：10.1038/nsmb.2516

• DNA METHYLATION
• NUCLEAR EXPORT
• GENE
• PROTEIN
• VECTOR
• CANCER
• EXPRESSION
• APOPTOSIS
• PATHWAY
• COMPLEX

PTEN is a tumor-suppressor gene that has been shown to be under the regulatory control of a PTEN pseudogene expressed noncoding RNA, PTENpg1. Here, we characterize a previously unidentified PTENpg1-encoded antisense RNA (asRNA), which regulates PTEN transcription and PTEN mRNA stability. We find two PTENpg1 asRNA isoforms, alpha and beta. The alpha isoform functions in trans, localizes to the PTEN promoter and epigenetically modulates PTEN transcription by the recruitment of DNA methyltransferase 3a and Enhancer of Zeste. In contrast, the beta isoform interacts with PTENpg1 through an RNA-RNA pairing interaction, which affects PTEN protein output through changes of PTENpg1 stability and microRNA sponge activity. Disruption of this asRNA-regulated network induces cell-cycle arrest and sensitizes cells to doxorubicin, which suggests a biological function for the respective PTENpg1 expressed asRNAs.

• 出版日期2013-4