DROSOPHILA STIMULATORY G-PROTEIN ALPHA-SUBUNIT ACTIVATES MAMMALIAN ADENYLYL CYCLASE BUT INTERACTS POORLY WITH MAMMALIAN RECEPTORS - IMPLICATIONS FOR RECEPTOR-G-PROTEIN INTERACTION

作者：QUAN F; THOMAS L; FORTE M

来源：Proceedings of the National Academy of Sciences of the United States of America, 1991, 88(5): 1898-1902.

DOI：10.1073/pnas.88.5.1898

• VACCINIA VIRUS EXPRESSION

Heterotrimeric guanine nucleotide binding proteins (G proteins) transduce signals from cell-surface receptors to intracellular effector proteins. Two forms of stimulatory G protein (G(s)) alpha-like subunit have been described in Drosophila melanogaster. To examine the function of these subunits we have used vaccinia virus vectors to express both proteins in cyc- cells, a murine S49 cell line deficient for G(s)alpha activity. Receptor-independent activation of each Drosophila G(s)alpha has demonstrated that both forms are capable of activating mammalian adenylyl cyclase and thus have the activity expected of stimulatory G proteins. However, the Drosophila G(s)alpha subunits interact poorly with mammalian G(s)-coupled receptors. These observations have helped to identify a region of high variability in G(s)alpha proteins that may be important for receptor interactions.

• 出版日期1991-3