AMPK isoform expression in the normal and failing hearts

作者：Kim Maengjo; Shen Mei; Ngoy Soeun; Karamanlidis Georgios; Liao Ronglih; Tian Rong^*

来源：Journal of Molecular and Cellular Cardiology, 2012, 52(5): 1066-1073.

DOI：10.1016/j.yjmcc.2012.01.016

• AMPK
• Mice
• Human
• Isoform expression
• Heart failure

AMP-activated protein kinase (AMPK) is a master metabolic switch that plays an important role in energy homeostasis at the cellular and whole body level, hence a promising drug target. AMPK is a heterotrimeric complex composed of catalytic alpha-subunit and regulatory beta- and gamma-subunits with multiple isoforms for each subunit. It has been shown that AMPK activity is increased in cardiac hypertrophy and failure but it is unknown whether changes in subunit composition of AMPK contribute to the altered AMPK activity. In this study, we determined the protein expression pattern of AMPK subunit isoforms during cardiac development as well as during cardiac hypertrophy and heart failure in mouse heart. We also compared the findings in failing mouse heart to that of the human failing hearts in order to determine whether the mouse heart is a good model of AMPK in human diseases. In mouse developmental hearts, AMPK was highly expressed in the fetal stages and fell back to the adult level after birth. In the failing mouse heart, there was a significant increase in alpha 2, beta 2, and gamma 2 subunits both at the mRNA and protein levels. In contrary, we found significant increases in the protein level of alpha 1, beta 1 and gamma 2c subunits in human failing hearts with no change in the mRNA level. We also compared isoform-specific AMPK activity in the mouse and human failing hearts. Consistent with the literature, in the failing mouse heart, the alpha 2 complexes accounted for similar to 2/3 of total AMPK activity while the alpha 1 complexes accounted for the remaining 30-35%. In the human hearts, however, the contribution of alpha 1-AMPK activity was significantly higher (>40%) in the non-failing hearts, and it further increased to 50% in the failing hearts. Thus, the human hearts have a greater amount of alpha 1-AMPK activity compared to the rodent hearts. In summary, the protein level and the isoform distribution of AMPK in the heart change significantly during normal development as well as in heart failure. These observations provide a basis for future development of therapeutic strategies for targeting AMPK.

• 出版日期2012-5