ADVENTITIOUS SHOOT REGENERATION AND MICROPROPAGATION OF CHIRITA FLAVIMACULATA W. T. WANG, C. EBURNEA HANCE, AND C. SPECIOSA KURZ.

作者：Nakano Masaru^*; Takagi Hiroki; Sugawara Shintaro; Saito Tomoka; Watanabe Yusuke; Lu Yuanxue; Guan Kaiyun; Godo Toshinari

来源：Propagation of Ornamental Plants, 2009, 9(4): 216-222.

• Chirita
• flow cytometry analysis
• histological observation
• plant regeneration
• ploidy stability
• rooting

Chirita flavimaculata, C. eburnea, and C. speciosa, which belong to Gesneriaceae, have high ornamental values such as beautiful foliages and showy flowers. An efficient plant regeneration system via adventitious shoot formation was established for micropropagation of these species. For all species, adventitious shoots elongating over 5 mm in length were efficiently regenerated from leaf explants on half-strength MS medium containing 0.1 mg l(-1) of both NAA and BA (8.1-15.6 shoots per explant). Histological observation indicated that adventitious shoot regeneration on a medium containing 0.1 mg l(-1) of both NAA and BA occurred directly from leaf epidermal cells without callus formation. Plantlets were obtained by rooting the shoots on half-strength MS medium containing 0.1 mg l(-1) IBA. No variations in the ploidy level were observed in the regenerated plants of three Chirita species as indicated by flow cytometry analysis.

• 出版日期2009-12
• 单位中国科学院昆明植物研究所