Decreased miR-452 expression and its tumor suppressive function in human gastric cancer

作者：Gao Haide^*; Liu Peng; Yang Yang; Gao Fang

来源：International Journal of Clinical and Experimental Medicine, 2016, 9(8): 16078-16085.

• MiR-452
• gastric cancer
• prognosis
• invasion

Objective: microRNAs (miRs) have been proved to play important roles in the tumorigenesis and development of human gastric cancer (GC). Our study aims to investigate the expression and function of miR-452 in GC. Methods: miR-452 expression was detected in GC tissues and cell lines by using qRT-PCR. Association between miR-452 levels and clinicopathological factors and patient prognosis was also analyzed. MTT, flow cytometry, and transwell invasion and migration assays were used to investigate the role of miR-452 in the regulation of biological behaviors of GC cells. Results: MiR-452 was significantly down-regulated in GC cell lines and clinical specimens. Decreased miR-452 expression was significantly associated with larger tumor size, deeper invasion depth, positive lymph node metastasis, and advanced clinical stage. Multivariate regression analysis identified low miR-452 expression as an independent predictor of poor survival. Overexpression of miR-452 inhibited GC cell proliferation, invasion and migration, and promoted cell apoptosis in vitro, while down-regulation of miR-452 showed the opposite effect. Conclusions: These findings indicate that miR-452 may act as a tumor suppressor in GC and would serve as a novel therapeutic agent for miR-based therapy.

• 出版日期2016
• 单位中国人民武装警察部队学院