A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar

作者：Coban Ahmet Yilmaz^*; Cayci Yeliz Tanriverdi; Deveci Aydin; Akgunes Alper; Uzun Meltem; Durupinar Belma

来源：Memorias do Instituto Oswaldo Cruz, 2011, 106(3): 378-380.

DOI：10.1590/S0074-02762011000300022

• Mycobacterium tuberculosis
• nitrate reductase assay
• blood agar

The susceptibility of 49 Mycobacterium tuberculosis clinical isolates to isoniazid (INH) and rifampisin (RIF) (28 multi-drug resistant-tuberculosis samples) was determined by a nitrate reductase assay (NRA) on blood agar. Agreement between the NRA and other testing methods was found to be 93.8% for both INH and RIF. The sensitivity, specificity, positive predictive value and negative predictive value for INH were 92.8%, 94.2%, 86.6% and 97%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value for RIF were 90.4%, 96.4%, 95% and 93.1%. In conclusion, we show here that blood agar can be used effectively for the NRA test.

• 出版日期2011-5