Lanthanum-containing bioparticles are associated with the influence of lanthanum on high phosphate mediated bone marrow stromal cells viability

作者：Bai, Jing; Wang, Xiao-hong; Zhang, Chan-juan; Huang, Jian^*; Mueller, Werner E. G.

来源：Biometals, 2018, 31(5): 771-784.

DOI：10.1007/s10534-018-0121-7

• Lanthanum-containing calcium phosphate particles
• High phosphate
• Bone marrow stromal cells
• Cells viability
• Cell apoptosis

Lanthanum (La)-based binder appears effective in reducing serum inorganic phosphate (Pi) levels among chronic dialysis patients, yet concern remains about La accumulation in bone during long-term oral administration. In this study, the effect of lanthanum chloride (LaCl3) on bone marrow stromal cells (BMSCs) viability was investigated under high Pi situation. We found low concentration (10(-9) M) of LaCl3 increased BMSCs viability, while high concentration (10(-5) M) of LaCl3 not only inhibited BMSCs viability but also stimulated high Pi induced cell apoptosis. In addition, La-containing calcium phosphate (CaP) particles can be detected on cell surfaces and inside cells. We also found that inhibition of CaP formation by adding sodium pyrophosphate, a recognized inhibitor of hydroxyapatite formation, abrogated LaCl3 induced the BMSCs viability. For isolated La-containing CaP particles, the particle size increased and crystal phase switched with elevated concentration of LaCl3. These results demonstrated that La-containing CaP particles were associated with the process of LaCl3 mediated BMSCs viability and the physicochemical properties of these particles played an important role in modulating BMSCs function.

• 出版日期2018-10
• 单位北京大学