Accumulation of a differentiation regulator specifies transit amplifying division number in an adult stem cell lineage

作者：Insco Megan L^*; Leon Arlene; Tam Cheuk Ho; McKearin Dennis M; Fuller Margaret T

来源：Proceedings of the National Academy of Sciences, 2009, 106(52): 22311-22316.

DOI：10.1073/pnas.0912454106

• bam
• spermatogenesis
• Drosophila
• transit amplifying cell division

A key feature of many adult stem cell lineages is that stem cell daughters destined for differentiation undergo several transit amplifying (TA) divisions before initiating terminal differentiation, allowing few and infrequently dividing stem cells to produce many differentiated progeny. Although the number of progenitor divisions profoundly affects tissue (re) generation, and failure to control these divisions may contribute to cancer, the mechanisms that limit TA proliferation are not well understood. Here, we use a model stem cell lineage, the Drosophila male germ line, to investigate the mechanism that counts the number of TA divisions. The Drosophila Bag of Marbles (Bam) protein is required for male germ cells to cease spermatogonial TA divisions and initiate spermatocyte differentiation [McKearin DM, et al. (1990) Genes Dev 4: 2242-2251]. Contrary to models involving dilution of a differentiation repressor, our results suggest that the switch from proliferation to terminal differentiation is triggered by accumulation of Bam protein to a critical threshold in TA cells and that the number of TA divisions is set by the timing of Bam accumulation with respect to the rate of cell cycle progression.

• 出版日期2009-12-29