A trap for in situ cultivation of filamentous actinobacteria

作者：Gavrish Ekaterina; Bollmann Annette; Epstein Slava; Lewis Kim^*

来源：Journal of Microbiological Methods, 2008, 72(3): 257-262.

DOI：10.1016/j.mimet.2007.12.009

• trap
• in situ cultivation
• filamentous actinobacteria

The approach of growing microorganisms in situ, or in a simulated natural environment is appealing, and different versions of it have been described by several groups. The major difficulties with these approaches are that they are not selective for actinomycetes - a group of gram-positive bacteria well known as a rich source of antibiotics. In order to efficiently access actinomycetes, a trap for specifically capturing and cultivating these microorganisms in situ has been developed, based on the ability of these bacteria to form hyphae and penetrate solid environments. The trap is formed by two semi-permeable membranes (0.2-0.6 mu m pore-size bottom membrane and 0.03 mu m pore-size top membrane) glued to a plastic washer with sterile agar or gellan gum inside. The trap is placed on top of soil, and filamentous microorganisms selectively penetrate into the device and form colonies. Decreasing the size of the pores of the lower membrane to 0.2 mu m restricted penetration of fungi. The trap produced more filamentous actinobacteria, and a higher variety of them, as compared to a conventional Petri dish cultivation from the same soil sample. Importantly, the trap cultivation resulted in the isolation of unusual and rare actinomycetes.

• 出版日期2008-3
• 单位西北大学