Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis

作者：Huang Fuqiang; Dang Zhisheng; Suzuki Yutaka; Horiuchi Terumi; Yagi Kinpei; Kouguchi Hirokazu; Irie Takao; Kim Kyeongsoon; Oku Yuzaburo^*

来源：PLoS Neglected Tropical Diseases, 2016, 10(4): e0004634.

DOI：10.1371/journal.pntd.0004634

Alveolar echinococcosis is a worldwide zoonosis of great public health concern. Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the egg and early larval stages. To address this information gap, we used a Next-Generation Sequencing approach to investigate three different stages (non-activated and activated oncospheres, and early stage metacestodes) of E. multilocularis (Nemuro strain). Transcriptome data analysis revealed that some diagnostic antigen gp50 isoforms and the antigen Eg95 family dominated in activated oncospheres, and the antigen B family dominated in early stage metacestodes. Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages. The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

• 出版日期2016-4
• 单位中国疾病预防控制中心; 中国疾病预防控制中心寄生虫病预防控制所