Development of 12 microsatellite markers in Euptelea polyandra by a random tailed genome-walking method using Phi29 DNA polymerase

作者：Wu Bingyun^*; Kurokochi Hiroyuki; Hogetsu Taizo

来源：Conservation Genetics Resources, 2009, 1(1): 59-61.

DOI：10.1007/s12686-009-9014-y

• Microsatellite (SSR)
• Euptelea polyandra
• Random tailed genome-walking
• Phi29 DNA polymerase

Twelve microsatellite (simple sequence repeat, SSR) markers were isolated from Euptelea polyandra, a Japanese endemic deciduous tree species, based on a random tailed genome-walking method using Phi29 DNA polymerase. There were 2 to 11 alleles per locus, and the observed and expected heterozygosities ranged from 0.05 to 0.90 and from 0.233 to 0.883, respectively.

• 出版日期2009-12