A study of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-alpha) serum levels in rats subjected to fecal peritonitis and treated with intraperitoneal ropivacaine

作者：Brocco Marcos Celio^*; Salomao Paulo Danilo Nagib; David de Almeida Carlos Eduardo; Carraretto Antonio Roberto; Cabral Sigmar Aurea; de Oliveira Silveira Amanda Cardoso; Gomez Renato Santiago; de Abreu Baptista Joao Florencio

来源：Acta Cirurgica Brasileira, 2012, 27(7): 494-498.

DOI：10.1590/S0102-86502012000700011

• Peritonitis
• Anesthesia
• Sepsis
• IL-6
• TNF-alpha

PURPOSE: The objective of this study was to assess the cytokine serum levels of IL-6 and TNF-alpha in rats subjected to fecal peritonitis and treated with peritoneal lavage with 0.2% ropivacaine by peritoneal lavage. %26lt;br%26gt;METHODS: We subjected 16 Wistar rats to laparotomy 6 hours after the induction of fecal peritonitis with autogenous stool and subsequently divided the rats randomly into 4 groups: I-control, no treatment; II-drying of the abdominal cavity; III-lavage of the abdominal cavity with 3 mL of 0.9% normal saline and drying; IV-lavage of the abdominal cavity with 3 mL of 0.2% ropivacaine and drying. Six hours following the laparotomy, the animals underwent cardiac puncture, and 1 mL of blood was collected for cytokine assessment before the animals were euthanized. %26lt;br%26gt;RESULTS: The lavage with ropivacaine resulted in smaller TNF-alpha levels compared with those observed in the other treatment groups (p %26lt;0.05). Regarding IL-6, the ropivacaine group showed lower cytokine levels than those observed in groups I and II, but there was no significant difference (p%26gt; 0.05) between groups III and IV. %26lt;br%26gt;CONCLUSION: Peritoneal lavage with 0.2% ropivacaine was shown to reduce plasma levels of IL-6 and TNF-alpha in the treatment of fecal peritonitis in rats.

• 出版日期2012-7