科研之友
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摘要
Terrestrial snails are able to transform themselves into inactivity ceasing their behavioral activity under unfavorable environmental conditions In the present study we report on the activity-dependent changes of the peptide and/or polypeptide profile in the brain and hemolymph of the snail Helix pomatia using MALDI TOF and quadrupole mass spectrometry The present data indicate that the snails respond to low temperature by increasing or decreasing the output of selected peptides Average mass spectra of the brain and hemolymph revealed numerous peaks predominantly present during the active state (19 and 10 peptides/polypeptides respectively) while others were observed only during hibernation (11 and 13) However there were peptides and/or polypeptides or their fragments present irrespective of the activity states (49 and 18) The intensity of fourteen peaks that correspond to previously identified neuropeptides varied in the brain of active snails compared to those of hibernating animals Among those the intensity of eight peptides increased significantly in active animals while in hibernated animals the intensity of another six peptides increased significantly A new peptide or peptide fragment at m/z 1110 7 was identified in a brain of the snail with the following suggested amino acid sequence GSGASGSMP-ATTS This peptide was found to be more abundant in active animals because the intensity of the peptide was significantly higher compared to hibernating animals In summary our results revealed substantial differences in the peptid
- 出版日期2010-12
