Acetylcholinesterase Biosensor for Chlorpyrifos Detection Based on Multi-Walled Carbon Nanotubes-SnO2-chitosan Nanocomposite Modified Screen-Printed Electrode

作者：Chen, Dongfei^*; Jiao, Yancui; Jia, Huiying; Guo, Yemin; Sun, Xia; Wang, Xiangyou; Xu, Jianguang

来源：International Journal of Electrochemical Science, 2015, 10(12): 10491-10501.

DOI：10.1016/s1452-3981(23)11274-0

• biosensor
• multi-walled carbon nanotubes
• tin oxide
• acetylcholinesterase

An acetylcholinesterase biosensor for organophosphate detection was developed immobilizing the AChE enzyme via MWCNTs-SnO2-CHIT nanocomposite on screen-printed electrode. MWCNTs provide a flexible conductive film and a much larger pathway due to their high electrical conductivity and large porosity ratio, thus increasing detection sensitivity. SnO2 decreased the peak voltage, revealed that the SnO2 nano materials can promote the redox process. Based on the inherent conductive properties of the MWCNTs-SnO2-CHIT, the immobilized AChE had greater affinity for ATCl and excellent catalytic effect in the hydrolysis of ATCl. Under optimized conditions, the proposed AChE biosensor exhibited sensitive and stable response for the detection of chlorpyrifos, ranging from 0.05 to 1.0x10(3) mu g/L with a limit of detection down to 0.05 mu g/L. The proposed biosensor was successfully applied in the determination of chlorpyrifos pesticides in cabbage, lettuce, leek and pakchoi samples, obtained acceptable recovery of 89.3 similar to 103.3%. With excellent stability, sensitivity, and simplicity, the proposed AChE biosensor showed a feasible quantitative method in detection of chlorpyrifos residues.

• 出版日期2015-12
• 单位山东理工大学