Development of 10 microsatellite markers for the Atlanto-Mediterranean sponge Petrosia ficiformis

作者：Taboada Sergi^*; Riesgo Ana; Blasco Gema; Sola Joan; Xavier Joana R; Lopez Legentil Susanna

来源：Conservation Genetics Resources, 2015, 7(4): 895-897.

DOI：10.1007/s12686-015-0496-5

• Porifera
• Conservation genetics
• Pyrosequencing
• Disease outbreak

Due to anthropogenic disturbances, the common sponge Petrosia ficiformis has suffered from severe disease outbreaks coincidental with episodic raises in seawater temperature. Here, we report on the optimization of ten microsatellites that can be used to estimate population connectivity and structure. This information will be critical for further conservation efforts across P. ficiformis' Atlanto-Mediterranean distribution. Microsatellites were isolated by genomic pyrosequencing and tested in two populations from the Mediterranean and Eastern Atlantic, each with 27 and 16 individuals, respectively. The allele number per locus ranged from 4 to 12, observed heterozygosity from 0.399 to 0.686, and expected heterozygosity from 0.421 to 0.715. No linkage disequilibrium between pairs of loci was detected.

• 出版日期2015-12