An Electrochemical Immunosensor for the Determination of Ractopamine Based on Multi-walled Carbon Nanotubes and Ionic Liquid Composite

作者：Chen Changyun^*; Zhang Honglin; Liu Minsheng; Yan Yan; Zhao Bo

来源：Acta Chimica Sinica, 2011, 69(23): 2865-2869.

• multi-walled carbon nanotube
• ionic liquid
• ractopamine
• electrochemical immunosensor

We have developed a new method of electrochemical immunosensor for the determination of ractopamine (RAC). The modified electrodes were prepared as follows. First, the multi-walled carbon nanotubes and ionic liquid composites were cast on glassy carbon electrode (GCE), and then the ractopamine-bovine serum albumin antigens were cast on GCE. At last, we dipped a few Nafion onto the electrode. Cyclic voltammetry and differential pulse voltammetry were employed to monitor the fabrication steps of iminunoreaction system in solutions containing K3Fe(CN)(6) as redox probes. The immunosensor demonstrates excellent RAC detection features with a broad linear range of 1 to 1500 ng/mL and a detection limit of 0.3 ng/mL. The tests with spiked animal feed samples showed excellent recovery for ractopamine.

• 出版日期2011-12-14
• 单位南京晓庄学院; 南京师范大学