Arhodomonas recens sp nov., a halophilic alkane-utilizing hydrogen-oxidizing bacterium from the brines of flotation enrichment of potassium minerals

作者：Saralov A I^*; Kuznetsov B B; Reutskikh E M; Baslerov R V; Panteleeva A N; Suzina N E

来源：Microbiology, 2012, 81(5): 582-588.

DOI：10.1134/S002626171205013X

• Arhodomonas
• new halophilic microorganism
• phylogeny
• alkane-utilizing bacteria
• hydrogen-oxidizing bacteria
• technogenic brines

A halophilic nonpigmented rod-shaped (0.8-1.0 x 2.0-2.5 mu m), gram-negative bacterium with a single polar flagellum (strain RS91) was isolated from acidic brines of flotation enrichment of potassium minerals (Silvinit Co., Solikamsk, Russia). The strain grew in the media with 2 to 25% NaCl (optimum at 10-12%), 20-45A degrees C (optimum at 37A degrees C), and pH 5.5-8.5 (optimum 6.5-7.5). It was an aerobe or facultative anaerobe incapable of fermentation. The strain was characterized by the absence of growth on glucose, fructose, and citrate, extensive aerobic growth on n-hexadecane and in the mineral medium with H-2 + O-2 + CO2 in the gas phase, anaerobic nitrate reduction with acetate or hydrogen (under H-2 + CO2 + N-2), and variable fatty acid composition. The DNA G+C content was 68.2 mol %. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that while strain RS91 was most closely related to Arhodomonas aquaeolei HA-1(T) (98.3%) and Nitrococcus mobilis (98.1%), it was only remotely related to the halophilic phototroph Halorhodospira halophila (90.6%). Based on the combination of its phenotypic and genotypic characteristics, the organism was classified as a new species of the genus Arhodomonas, family Ectothiorhodospiraceae with the proposed name Arhodomonas recens sp. nov. The type strain is RS91(T) (= IEGM 796(T) = VKPM B-11280(T)).

• 出版日期2012-9