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摘要
Deoxynivalenol (DON) is a frequently found trichothecene mycotoxin that elicits toxic effects on humans and animals. In pigs, DON induces changes in digestive and immune systems. Effects on the reproductive system are scarce and mainly based in in vitro models. The aim of this study was to evaluate, using an ex vivo model, the effects of DON on the morphology of ovaries of pigs in all stages of follicular development. Six 5-month-old pigs were used for sampling the explants. Thirty-six explants were incubated for 48 hours in culture medium (n = 18) or medium containing 10 mu M of DON (n = 18). After the incubation period, the explants were submitted to histologic and immunohistochemical (proliferating cell nuclear antigen [PCNA] expression) analysis. Histologic changes were scored, and a lesional score was established. Oocytes and follicular cells immunostained for PCNA were counted. Explants exposed to DON showed a significant increase in the lesional score (P = 0.0004) compared to control explants. The main histologic changes were degeneration of oocytes and granulosa cells, interstitial edema and pyknotic cells. DON induced a reduction in the number of normal follicles in all stages of follicular development: primordial (P = 0.005), primary (P = 0.04), and growing follicles (P = 0.04) compared to control group. Deoxynivalenol also induced a significant increase (P <= 0.05) in the number of pyknotic oocytes in all stages of follicular development; however, no significant change in PCNA expression in oocytes or follicular cells was observed. These results indicated that DON induces toxic effects on the ovaries, affecting follicular development and interfering with reproductive parameters on pigs. Also, the present data indicate that ovarian explants are an adequate model for assessing reproductive toxicity.
- 出版日期2017-3-1
