Discrimination Cascade Enabled Selective Detection of Single Nucleotide Mutation

作者：Li, Lidan; Xiao, Xianjin; Ge, Jingyang; Han, Manli; Zhou, Xu; Wang, Lei; Su, Xin^*; Yu, Changyuan^*

来源：ACS Sensors, 2017, 2(3): 419-425.

DOI：10.1021/acssensors.7b00005

• single nucleotide mutation
• toehold strand displacement
• endonuclease IV
• single molecule analysis
• cancer diagnosis

Owing to the significance of single nucleotide mutation (SNM) for personalized medicine, the detection of SNM with high accuracy has recently attracted considerable interest. Here, we present a kinetic method for selective detection of SNM based on a discrimination cascade constructed by combining the toehold strand displacement (TSD) and endonuclease IV (Endo IV) catalyzed hydrolysis: The single -nucleotide specificity of the two DNA reactions allows highly selective detection of all types of single nucleotide changes (including single-nucleotide insertion and deletion), achieving a high discrimination factor with a median of 491 which is comparable with recently reported methods. For the first time, the enzyme assisted nucleic acid assay was characterized by single molecule analysis on total internal reflection fluorescence microscope (TIRFM) suggesting that the two steps do not work independently and the rate of TSD can be tuned by Endo IV facilitated conformation selection. The effective discrimination of the point mutation of BRAF gene in cancer and normal cell line suggests that this method can be a prominent post-PCR genotyping assay.

• 出版日期2017-3
• 单位华中科技大学; 北京化工大学