A New Ochratoxin A Biodegradation Strategy Using Cupriavidus basilensis Or16 Strain

作者：Ferenczi Szilamer^*; Cserhati Matyas; Krifaton Csilla; Szoboszlay Sandor; Kukolya Jozsef; Szoke Zsuzsanna; Koszegi Balazs; Albert Mihaly; Barna Terez; Mezes Miklos; Kovacs Krisztina J; Kriszt Balazs

来源：PLos One, 2014, 9(10): e109817.

DOI：10.1371/journal.pone.0109817

Ochratoxin-A (OTA) is a mycotoxin with possibly carcinogenic and nephrotoxic effects in humans and animals. OTA is often found as a contaminant in agricultural commodities. The aim of the present work was to evaluate OTA-degrading and detoxifying potential of Cupriavidus basilensis OR16 strain. In vivo administration of OTA in CD1 male mice (1 or 10 mg/kg body weight for 72 hours or 0.5 mg/kg body weight for 21 days) resulted in significant elevation of OTA levels in the blood, histopathological alterations- and transcriptional changes in OTA-dependent genes (annexinA2, clusterin, sulphotransferase and gadd45 and gadd153) in the renal cortex. These OTA-induced changes were not seen in animals that have been treated with culture supernatants in which OTA was incubated with Cupriavidus basilensis OR16 strain for 5 days. HPLC and ELISA methods identified ochratoxin a as the major metabolite of OTA in Cupriavidus basilensis OR16 cultures, which is not toxic in vivo. This study has demonstrated that Cupriavidus basilensis OR16 efficiently degrade OTA without producing toxic adventitious metabolites.

• 出版日期2014-10-10