摘要

The antioxidant activities of three beverages, coffee, black tea and green tea, along with their major components, were investigated in terms of their reaction with the stable radical 2,2'-dipheny1-2-picrylhydrazyl (DPPI-1(.)). We used a kinetic approach in parallel with quantification methods based on a fixed end-point to determine the scavenging efficiency of compounds abundant in these beverages during their reaction with DPPH. using a stopped-flow spectrophotometer-based method. Ascorbic acid, (+)-catechin, (-)-epigallocatechin, tannic acid, and caffeic acid were selected as model antioxidants to study in coffee, black tea and green tea. We applied a second-order model to demonstrate similarities in the kinetics behavior of beverages and related compounds. Our findings showed the slopes K-2'((mol/L)(-1) s(-1)) and K-max ((mol/L)(-1) s(-1)) exhibited similar and correlated values; we suggest the variation in k(2)' as a function of time is more informative about antioxidant properties than reaction with DPPH. alone. We also used IC100 to test the reliability of the relative

  • 出版日期2014-5-1