Systemic CD4+ and CD8+ T-cell cytokine profiles correlate with GOLD stage in stable COPD

作者：Paats Marthe S^*; Bergen Ingrid M; Hoogsteden Henk C; van der Eerden Menno M; Hendriks Rudi W

来源：European Respiratory Journal, 2012, 40(2): 330-337.

DOI：10.1183/09031936.00079611

• Chronic obstructive pulmonary disease
• cytokine
• flow cytometry
• T-helper 17
• T-lymphocytes

Chronic obstructive pulmonary disease (COPD) is associated with pulmonary and systemic inflammation. Both CD4+ and CD8+ T-lymphocytes play a key role in COPD pathogenesis, but cytokine profiles in circulating T-lymphocytes have not been well characterised.
Here we report the analysis of peripheral blood T-cells from 30 stable COPD patients and 10 healthy never-smokers for interferon (IFN)-gamma, interleukin (IL)-4, tumour necrosis factor (TNF)-alpha and the T-helper 17 cytokines IL-17A, IL-17F and IL-22 by intracellular flow cytometry.
We found significantly increased proportions of IFN-gamma+ and TNF-alpha+ CD8+ T-cells in COPD patients, when compared with healthy controls. This was most evident in patients with less severe disease. In contrast, expression profiles in circulating CD4+ T-cells were similar in COPD patients and healthy controls for all cytokines tested, except for IL-17F. COPD patients with more severely reduced diffusing capacity had lower proportions of IL-17A+ CD4+ T-cells. Proportions of IL-22+ cells in the CD4+ memory T-cell population were significantly increased in active smokers, when compared with past smokers.
Collectively, this comprehensive cytokine analysis of circulating T-cells in COPD patients revealed a correlation for CD8+ T-cells between Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage and IFN-gamma or TNF-alpha expression, but not for CD4+ T-cells.

• 出版日期2012-8