Alkaloids derived from flowers of Senna spectabilis, (-)-cassine and (-)-spectaline, have antiproliferative activity on HepG2 cells for inducing cell cycle arrest in G1/S transition through ERK inactivation and downregulation of cyclin D1 expression

作者：Pereira Rodrigo Machado; Ferreira Silva Guilherme Alvaro; Pivatto Marcos; Santos Luciana de Avila; Bolzani Vanderlan da Silva; Chagas de Paula Daniela Aparecida; de Oliveira Jaqueline Carvalho; Viegas Junior Claudio; Ionta Marisa^*

来源：Toxicology in Vitro, 2016, 31: 86-92.

DOI：10.1016/j.tiv.2015.11.018

• Hepatocellular carcinoma
• Cell cycle arrest
• (-)-Cassine
• (-)-Spectaline
• Piperidine alkaloids
• Senna spectabilis

Cancer is one of the most critical problems of public health in the world and one of the main challenges for medicine in this century. Unfortunately, most patients are diagnosed at advanced stage, when the treatment options are palliative. Consequently, the search for novel therapeutic options is imperative. In the context, the plants represent an important source for discovering of novel compounds with pharmacological potential including antineoplastic agents. Herein, we aimed to investigate in vitro antiproliferative and cytotoxic potentials of an alkaloid mixture derived from Senna spectabilis, (-)-cassine (1) and (-)-spectaline (2). These alkaloids reduced cell viability in a concentration-dependent manner of six tumor cell lines. From initial screening, HepG2 cells were selected for further investigations. We show that alkaloids 1/2 have an important antiproliferative activity on HepG2 cells due to their ability in inducing cell cycle arrest in G1/S transition. This effect was associated to ERIC inactivation and down-regulation of cyclin D1 expression. In addition, we evidenced a disruption of the microfilaments and microtubules in a consequence of the treatment. Taken together, the data showed by the first time that alkaloids 1/2 strongly inhibit cell proliferation of hepatocellular carcinoma cells. Therefore, they represent promise antitumor compounds against liver cancer and should be considered for further anticancer in vivo studies.

• 出版日期2016-3