DOG(R)1 AND DOG(R)2 - 2 GENES FROM SACCHAROMYCES-CEREVISIAE THAT CONFER 2-DEOXYGLUCOSE RESISTANCE WHEN OVEREXPRESSED

作者：RANDEZGIL F; BLASCO A; PRIETO JA; SANZ P

来源：Yeast, 1995, 11(13): 1233-1240.

DOI：10.1002/yea.320111303

• SACCHAROMYCES CEREVISIAE
• 2-DEOXYGLUCOSE
• 2-DEOXYGLUCOSE-6 PHOSPHATE PHOSPHATASE
• DOG(R)1
• DOG(R)2

Saccharomyces cerevisiae contains two genes (DOG(R)1 and DOG(R)2) that are able to confer 2-deoxyglucose resistance when they are overexpressed. These genes are very similar, sharing 92% identity at the protein level. They code for two isoenzymes with 2-deoxyglucose-6 phosphate (2-DOG-6P) phosphatase activity. These enzymes have been purified and characterized. Dog(R)1p shows an optimum pH of 6, an optimum temperature of 30 degrees C and a K-M on 2-DOG-6P of 17 mM. Dog(R)2p shows a similar optimum pH, but the optimum temperature is 40 degrees C and it exhibits a K-M on 2-DOG-6P of 41 mM. Both enzymes require 10 mM-MgCl2 for maximal activity and they are inhibited by inorganic phosphate.

• 出版日期1995-10