An iTRAQ based quantitative proteomic analysis to explore novel different proteins between nasopharyngeal carcinoma cell lines and their drug resistance cell lines

作者：WANG Kun; TAO Ya; WU Qiong; LONG Lu; CHEN Zhen; YI Bin

来源：第一次全国中西医结合检验医学学术会议暨中国中西医结合学会检验医学专业委员会成立大会, 2014-06-12.

• iTRAQ
• nasopharyngeal carcinoma
• cheoresistance
• bioinformatics

Purpose:To discover chemoresistant-associated proteins with the resistant model cell lines.Methods:we used the isobaric tags for relative and absolute quantitation(iTRAQ) approach combined with nano liquid chromatography-tandem mass spectrometry(NanoLC-MS/MS) analysis to identify proteins that were differentially expressed between nasopharyngeal carcinoma cell lines CNE1,CNE2 and their CDDP resistance cell lines CNE1 DDP and CNE2 DDP.Results:By three employing biological and technical replicates,we identified 817 nonredundant proteins,64 of which were differentially expressed in the two group cell lines(CNElvs.CNElDDP and CNE2 vs.CNE2DDP).Through the bioinformatics:Go analysis,KEGG pathway and Protein-protein Interaction of differentially expressed proteins.some proteins such as HSPB1,CLIC1,ANXA1,TPM4,Plastin-2,PDIA1 and so on,might play an important role in the mechanisms of chemoresistance and clinical theray.Conclusion:The present data first time show that the chemotherapy resistant protein spectrum through these 4 cell lines,and may excavate novel potential biomarkers for early detection of chemoresistance in nasopharyngeal carcinoma.

• 出版日期2014
• 单位中南大学