a -L-Rhamnosidase and ss -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis

作者：Alejandra Eliades Lorena; Lorena Rojas Natalia; Noemi Cabello Marta; Enrique Voget Claudio; Nazareno Saparrat Mario Carlos

来源：Journal of Basic Microbiology, 2011, 51(6): 659-665.

DOI：10.1002/jobm.201100163

• Alkaline enzymes
• Fungi
• a -L-Rhamnosidase
• ss -D-glucosidase
• Naringin hydrolysis

a -L-Rhamnosidases (EC 3.2.1.40) and beta -D-glucosidases (EC 3.2.1.21) obtained from several microbial sources are potential catalysts in food, beverage, and pharmaceutical industries. However, the enzyme preparations currently used have limitations related to the stability and activity of the enzyme as well to their reuse. A microtiter screening was carried out in 55 fungal strains isolated from alkaline soils, to obtain active a -L-rhamnosidases and beta -D-glucosidases at pH 9.0. While a -L-rhamnosidase activity was detected in 45% of the strains tested, beta -D-glucosidase activity was found only in 27%. Based on the fungal ability to produce a -L-rhamnosidase activity, cultures were supplemented with naringin to study the activities of the enzymes and the potential of the fungal strains on naringin hydrolysis. About 70% of the fungal strains tested increased the activities of both enzymes in the naringin-supplemented cultures as compared to non-supplemented ones. This effect was higher in Acrostalagmus luteo-albus LPSC 427 (15.3 fold) for a -L-rhamnosidase activity and Metarrhizium anisopliae LPSC 996 (51.1 fold) for beta -D-glucosidase activity. All the enzyme preparations tested hydrolyzed naringin at pH 9.0, being that obtained from Acremonium murorun LPSC 927 cultures the one which showed highest hydrolysis. Here, different fungal species are reported for the first time for their ability to produce a -L-rhamnosidase and beta -D-glucosidase activity at alkaline pH.

• 出版日期2011-12