A bis(2,2 '-Bipyridine) (Dipyrido[3, 2-a:2 ' 3 '-c]Phenazine-N4N5) Ruthenium(II)-Based Electrochemiluminescence Biosensor for Evaluation of DNA Damage

作者：Lu Liping^*; Wu Jing; Li Meng; You Xueying; Kang Tianfang; Cheng Shuiyuan

来源：Analytical Letters, 2015, 48(1): 116-126.

DOI：10.1080/00032719.2014.933432

• Electrochemiluminescence
• [Ru(bpy)(2)(dppz)](2+)
• DNA damage
• Perfluorooctanoic acid
• DNA charge transfer

The electrochemiluminescence of bis(2, 2 '-bipyridine) (dipyrido[3, 2-a:2 ' 3 '-c]phenazine-N4N5) ruthenium(II) ([Ru(bpy)(2)(dppz)](2+)) was used to monitor deoxyribonucleic acid (DNA) charge transfer with tri-n-propylamine as a coreactant. This system was used to measure damage to DNA induced by perfluorooctanoic acid. Fifteen-base pairs of double-stranded DNA with a thiol group at the 5 ' end position were covalently bonded to a gold electrode. An electrochemiluminescence sensor was then constructed by incubating the modified gold electrode in [Ru(bpy)(2)(dppz)](2+) solution for 30min. For comparison, single-stranded DNA, well-matched double-stranded DNA, and single base-mismatched double-stranded DNA were assembled on the gold surface. The results showed that the electrochemiluminescence behavior of the DNA sensors were unique. The electrochemiluminescence decreased when the [Ru(bpy)(2)(dppz)](2+)-DNA ECL sensor was incubated in a perfluorooctanoic acid solution. The damage to DNA caused by perfluorooctanoic acid was monitored using a combination of DNA charge transfer theory and the interaction between DNA and [Ru(bpy)(2)(dppz)](2+). The detection limit for perfluorooctanoic acid was 1x10(-12)mol/L. [Ru(bpy)(2)(dppz)](2+) was shown to be a sensitive electrochemiluminescence sensor for the determination of DNA damage.

• 出版日期2015-1-2
• 单位北京工业大学