Aneuploid yeast strains exhibit defects in cell growth and passage through START

作者：Thorburn Rebecca R; Gonzalez Christian; Brar Gloria A; Christen Stefan; Carlile Thomas M; Ingolia Nicholas T; Sauer Uwe; Weissman Jonathan S; Amon Angelika^*

来源：Molecular Biology of the Cell, 2013, 24(9): 1274-1289.

DOI：10.1091/mbc.E12-07-0520

Aneuploidy, a chromosome content that is not a multiple of the haploid karyotype, is associated with reduced fitness in all organisms analyzed to date. In budding yeast aneuploidy causes cell proliferation defects, with many different aneuploid strains exhibiting a delay in G1, a cell cycle stage governed by extracellular cues, growth rate, and cell cycle events. Here we characterize this G1 delay. We show that 10 of 14 aneuploid yeast strains exhibit a growth defect during G1. Furthermore, 10 of 14 aneuploid strains display a cell cycle entry delay that correlates with the size of the additional chromosome. This cell cycle entry delay is due to a delayed accumulation of G1 cyclins that can be suppressed by supplying cells with high levels of a G1 cyclin. Our results indicate that aneuploidy frequently interferes with the ability of cells to grow and, as with many other cellular stresses, entry into the cell cycle.

• 出版日期2013-5-1