科研之友
微信
新浪微博
Facebook
分享链接
摘要
Early osteoarthritis (OA) is poorly understood, but abnormal chondrocyte morphology might be important. We studied IL-1 beta and pericellular collagen type VI in morphologically normal and abnormal chondrocytes. In situ chondrocytes within explants from non-degenerate (grade 0/1) areas of human tibial plateaus (n = 21) were fluorescently labeled and visualized [2-photon laser scanning microscopy (2PLSM)]. Normal chondrocytes exhibited a "smooth" membrane surface, whereas abnormal cells were defined as demonstrating >1 cytoplasmic process. Abnormal chondrocytes were further classified by number and average length of cytoplasmic processes/cell. IL-1 beta or collagen type VI associated with single chondrocytes were visualized by fluorescence immuno-histochemistry and confocal laser scanning microscopy (CLSM). Fluorescence was quantified as the number of positive voxels (i.e., 3D pixels with fluorescence above baseline)/cell. IL-1 beta-associated fluorescence increased between normal and all abnormal cells in the superficial (99.7 +/- 29.8 [11 (72)] vs. 784 +/- 382 [15 (132)]; p = 0.04, positive voxels/cell) and deep zones (66.5 +/- 29.4 [9 (64)] vs. 795 +/- 224 [9 (56)]; p = 0.006). There was a correlation (r(2) = 0.988) between the number of processes/cell (0-5) and IL-1 beta and an increase particularly with short processes (<= 5 mu m; p = 0.022). Collagen type VI coverage and thickness decreased (p < 0.001 and p = 0.005, respectively) with development of processes. Abnormal chondrocytes in macroscopically nondegenerate cartilage demonstrated a marked increase in IL-1 beta and loss of pericellular type VI collagen, changes that could lead to cartilage degeneration.
- 出版日期2010-11
