Apoptotic index and histological assessment of hepatocytes after adriamycin treatment

作者：Boratynski Zbigniew^*; Pedrycz Agnieszka; Siermontowski Piotr; Kocki Janusz; Zajac Agata

来源：Medycyna Weterynaryjna, 2011, 67(7): 467-469.

• rats
• adriamycin
• hepatocytes
• apoptotic index
• histocytological analysis
• time

Adriamycin is an antineoplastic antibiotic which is biodegragated mainly in the liver. The purpose of this study was to analyse the apoptotic index and the histological structure of apoptotic cells in the rat liver. Rats were examined 4 and 7 weeks after adriamycin treatment. The animals were divided into four equal groups: groups I and HI, consisting of rats treated with a single intraperitoneal dose of adriamycin (5 mg/kg b.w) and decapitated after 4 or 7 weeks; groups II and IV comprising control rats treated with a single intraperitoneal dose of 0.9% NaCl (0.5 ml) and decapitated after 4 or 7 weeks. After decapitation, liver specimens were collected and embedded in paraffin. Slides were stained with haematoxylin and eosin. The degree of apoptosis was determined quantitatively using the apoptotic index. Statistical analysis was performed using the one-way ANOVA test and Student's t-test.
A single dose of adriamycin induced apoptosis of hepatocytes, which increased with time The apoptotic index was significantly higher in the group examined 7 weeks after adriamycin administration. The histological structure of the liver in the experimental groups was similar. The lesions observed were focal. The blurring of the hepatic cell membrane and a focal disintegration of architectonics concerning the shape and size of hepatocytes were visible. Chromatin in the nuclei was dispersed. Some nuclei showed peripheral chromatin condensation. The cytoplasm of some cells showed numerous vacuoles. Naked nuclei and congestion resulting from vessel damage were visible.

• 出版日期2011-7
• 单位河北医科大学