Ampelopsin attenuates brain aging of D-gal- induced rats through miR-34a-mediated SIRT1/mTOR signal pathway

作者：Kou, Xianjuan; Liu, Xingran; Chen, Xianbing; Li, Jie; Yang, Xiaoqi; Fan, Jingjing; Yang, Yi; Chen, Ning^*

来源：Oncotarget, 2016, 7(46): 74484-74495.

DOI：10.18632/oncotarget.12811

• ampelopsin
• aging
• autophagy
• miR-34a
• SIRT1-mTOR signal pathway
• Gerotarget

The underlying molecular mechanisms for aging-related neurodegenerative diseases such as Alzheimer's disease (AD) are not fully understood. Currently, growing evidences have revealed that microRNAs (miRNAs) are involved in aging and aging-related diseases. The up-regulation of miR-34a has been reported to be associated with aging-related diseases, and thus it should be a promising therapeutic target. Ampelopsin, also called dihydromyricetin (DHM), a natural flavonoid from Chinese herb Ampelopsis grossedentata, has been reported to possess multiple pharmacological functions including anti-inflammatory, anti-oxidative and anticancer functions. Meanwhile, it has also gained tremendous attention against neurodegenerative diseases as an anti-aging compound. In the present study, the model rats with D-gal-induced brain aging revealed an obvious expression of miR-34a; in contrast, it could be significantly suppressed upon DHM treatment. In addition, target genes associated with miR-34a in the presence of DHM treatment were also explored. DHM supplementation inhibited D-gal-induced apoptosis and rescued impaired autophagy of neurons in hippocampus tissue. Moreover, DHM activated autophagy through up-regulated SIRT1 and down-regulated mTOR signal pathways due to the down-regulated miR-34a. In conclusion, DHM can execute the prevention and treatment of D-gal-induced brain aging by miR-34a-mediated SIRT1-mTOR signal pathway.

• 出版日期2016-11-15
• 单位武汉体育学院; 湖北民族大学