Downstream processing of pullulan from fermentation broth

作者：Wu Shengjun; Jin Zhengyu^*; Kim Jin Moon; Tong Qunyi; Chen Hanqing

来源：Carbohydrate Polymers, 2009, 77(4): 750-753.

DOI：10.1016/j.carbpol.2009.02.023

• Aureobasidium pullulans
• Pullulan
• Downstream processing

pullulan, a water soluble extracellular polysaccharide, was produced by downstream fermentation employing the strain Aureobasidium pullulans. To obtain pure biopolymer from the fermentation broth, it is necessary to harvest cells, heat the broth, remove the melanin pigments co-produced during fermentation, concentration, precipitate and dry. Centrifugation of the fermentation broth at 10,000 rpm for 15 min gave cell pellets that were discarded and a green-black supernatant containing melanin pigment was subjected to the heat treatment at 80 degrees C for 20 min in order to remove the protein in the fermentation broth. The supernatant was demelanized by oxidation with hydrogen peroxide, concentrated under vacuum. precipitated with ethanol and dried at 60 degrees C for 30 min. This procedure produced high purity pullulan that was comparable in color and texture to the commercial samples.

• 出版日期2009-7-19
• 单位江南大学