Analysis and characterization of expressed sequence tag (EST) and simple sequence repeats (SSR) from red sea bream, Chrysophrys major, and gilthead sea bream, Sparus aurata

作者：Liu, Yun-Guo^*; Chen, Song-Lin; Liu, Chun-Ying; Li, Fang-Zheng

来源：Israeli Journal of Aquaculture – Bamidgeh, 2008, 60(2): 75-81.

• red sea bream
• gilthead sea bream
• EST
• SSR
• microsatellite

A total of 101 and 119 simple sequence repeats (SSR) were obtained from expressed sequence tags (EST) of the red sea bream (Chrysophrys major) and gilthead sea bream (Sparus aurata), respectively. Dimeric repeats were the most abundant class of SSR in both red (70.30%) and gilthead (51.26%) sea bream. Trimeric, tetrameric, and pentameric repeats occurred in decreasing proportions, i.e., 21.36%, 6.8%, and 0.97% in red sea bream and 45.38%, 3.36%, and 0 in gilthead. There were no hexameric repeats in EST of either species. The frequency of every class of SSR (dimeric, trimeric, tetrameric, and pentameric) decreased with increasing repeat length. In cross-species amplification of 20 existing microsatellite loci from gilthead sea bream in 20 red sea bream specimens, twelve loci showed positive amplification and four loci showed polymorphic amplification with two or three alleles.

• 出版日期2008-6
• 单位中华人民共和国山东出入境检验检疫局; 青岛农业大学; 中国海洋大学; 中国水产科学研究院