A Set of Allele-specific Markers Linked to L Locus Resistant to Tobamovirus in Capsicum spp.

作者：Lee Jundae; Han Jung Heon; Yoon Jae Bok^*

来源：Korean Journal of Horticultural Science and Technology, 2012, 30(3): 286-293.

DOI：10.7235/hort.2012.12018

• CAPS
• L-3
• L-4
• PMMoV
• SCAR

The resistance to Tobamovirus in Capsicum spp. has been known to be controlled by five different alleles (L-0, L-1, L-2, L-3, and L-4) of L locus on the telomere of long arm of pepper chromosome 11. To develop a set of molecular markers differentiating all the alleles of L locus, we used five pepper differential hosts including Capsicum annuum Early California Wonder (ECW, (LL0)-L-0), C. annuum Tisana ((LL1)-L-1), C. annuum Criollo de Morelos 334 (CM334, (LL2)-L-2), Capsicum chinense PI 159236 ((LL3)-L-3), and Capsicum chacoense PI 260429 ((LL4)-L-4). Developing a series of CAPS or SCAR markers specifically linked to the alleles was allowed by the sequence comparison of PCR amplicons of the L-3-linked markers (189D23M, A339, and 253A1R) and BAC sequences (FJ597539 and FJ597541) in the pepper differentials. Genotypes deduced by these markers in 48 out of 53 F-1 hybrids of commercial pepper varieties were consistent with their phenotypes by bioassay using Tobamovirus pathotypes (P-0, P-1, and P-1,P-2). Consequently, these markers can be useful to differentiate L alleles and for breeding Tobamovirus resistance in pepper with marker-assisted selection.

• 出版日期2012-6