Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes

作者：Silva Pereira Luciana Lopes; Marcussi Silvana; Satiro Livia Cabral; Pereira Chrystian Araujo; Andrade Larissa Fonseca; Davide Lisete Chamma; dos Santos Custodio Donizete

来源：Brazilian Journal of Pharmaceutical Sciences, 2012, 48(1): 103-108.

• Genotoxicity
• White bean flour/genotoxic effects
• Comet assay/toxicological analysis of food
• Food/toxicological analysis

This study was conducted to evaluate the potential induction of genotoxic effects of white bean flour using the Comet assay. The test was conducted with human lymphocytes present in whole blood immediately after collection, by incubation with white bean flour in three concentrations (3.92, 9.52 and 18.18 mg/mL) at 37 degrees C for 411 followed by preparation of slides. Samples were considered positive (above 20% damage) when the damage observed to cellular DNA was higher than the negative control. No genotoxic potential was found at the doses tested. However, it would be premature to suggest absence of risk to human health of DNA damage since the exposure of cells to the extract was restricted to four hours rather than a whole cell cycle. Additionally, further information on toxicology should be obtained in future studies.

• 出版日期2012-3